|Metabolite analysis by 1H-NMR spectroscopy
1H-NMR spectroscopy is the method of choice for the analysis of extra-cellular metabolites in the milli- to micro- molar range. Typical questions in this area are the uptake by cells in tissue culture of components of the culture medium or the release of metabolites from the cells.
The advantage of the 1H-NMR spectroscopy is that the preparation of the probe is straightforward since no derivatisation is needed as is in general the case for GC-MS. Furthermore the analysis of a sample by 1H-NMR spectroscopy allows for the simultaneous quantitation of 30 to 50 different metabolites. One final advantage of the method is that it permits the quantitation of all metabolites using just one single standard as spike so that the time consuming process of constructing calibration curves is obviated.
Sample volume: 500 - 1000 µl
Metabolite concentration: in the milli-molar to micro-molar range
The sample must fulfil the following criteria: