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Special NMR-methods - Sample need and measurement time

Information about required sample amounts applies to pure substances with a molecular mass below 500g/mol. For compounds with higher molecular weight the time required for analysis will be proportionally longer.

Information about sample amounts for the NMR-spectroscopic characterisation of substances in mixtures (natural extracts, samples from stress tests, synthetic mixtures etc.) by LC-SPE-NMR/MS are shown at the appropriate site.

 

 

1H-NMR

 

Measurement in 5mm-NMR-tubes

 

Sample amount  

1 - 2 mg

0.5 - 1 mg

  analysis time about   5 min

  analysis time about 30 min

 

 

Measurement in 30 µl sample cell

 

Sample amount  

20 - 100 µg

10 - 20 µg

  analysis time about 15 min

  analysis time about 30 min

 

 

13C-NMR

 

Measurement in 5mm-NMR-tubes

 

Sample amount  

10 - 20 mg

5 - 10 mg

2 - 5 mg

1 - 2 mg

  analysis time about   2 -   3 hours

  analysis time about   6 - 12 hours

  analysis time about 12 - 24 hours

  analysis time about 24 - 48 hours

 

 

1H-1H-COSY; 1H-1H-NOESY und 1H-1H-ROESY

 

Note: analysis times given are for the generation of one spectrum

 

Measurement in 5mm-NMR-tubes

 

Sample amount  

5 - 10 mg

2 - 5 mg

1 - 2 mg

    analysis time about   2 -   4 hours

    analysis time about   6 - 12 hours

    analysis time about 12 - 24 hours

 

 

Measurement in 30 µl sample cell

 

Sample amount  

50 - 100 µg

20 -   50 µg

  analysis time about   6 - 12 hours

  analysis time about 12 - 24 hours

 

 

1H-13C-HSQC bzw. 1H-13C-HMBC

 

Note: analysis times given are for the generation of one spectrum

 

Measurement in 5mm-NMR-tubes

 

Sample amount  

5 - 10 mg

2 -   5 mg

1 -   2 mg

    analysis time about   4 -   6 hours

    analysis time about   6 - 12 hours

    analysis time about 12 - 24 hours

 

 

Measurement in 30 µl sample cell

 

Sample amount 

100 - 200 µg

50 - 100 µg

  analysis time about   5 - 12 hours

  analysis time about 12 - 24 hours

 

 

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